产品货号：
GS2181
中文名称：
FokI内切酶
英文名称：
FokI
产品规格：
200U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
Overdigestions of>5 units of Fok I per 1μgof DNA and incubation times>2hoursare not recommended.
技术规格
相关搜索：FokI内切酶，FokI限制性内切酶
使用说明
Overdigestions of>5 units of Fok I per 1μgof DNA and incubation times>2hoursare not recommended.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | GGATG(N)9↑ |
| 来源 | E.coli strain that carries the cloned Fok I gene from Flavobacterium okeanokoites |
| Assayed on | Lambda DNA |
| Optimal buffer | Y?(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.) |
| Optimal temperature | 37℃ |
| Storage conditions | 10 mM Tris-HCl(pH 7.5);50 mM NaCl;0.1 mM EDTA;1 mM DTT;200 μg/ml BSA;50%glycerol;Store at -20℃. |
| Ligations | After 2-fold overdigestion with enzyme more than 95%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 1 u.a.of enzyme for 16 hours at 37℃ |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
| Campatible Ends | BseGI^,BstF5I^,BtsCI^ |
相关搜索：FokI内切酶，FokI限制性内切酶